Imaging extracellular neuronal signaling on high resolution microelectrode arrays (MEAs) Hippocampal cultures coupled with a high resolution neuroelectronic interface

This paper describes the experimental performances of a high-resolution CMOS integrated microelectrode array platform for acquiring extracellular neuronal signaling in-vitro. Electrophysiological signals of dissociated hippocampal cultures (14-30 DIVs, E18 from rats) are sampled at 7.7 kHz/channel from 4096 microelectrodes (21 mum inter-electrode separation). We show that the achieved spatial-temporal resolution together with data visualization as image sequences, enable the detailed observation of activation sites and burst activity propagations. This image-based approach is well adapted to high-resolution datasets in respect to raster plot visualization and opens the perspective of detailed image-based analysis of extracellular neuronal signals.