Ultrafast gain dynamics of the green fluorescent protein

Author

Didier ; Guidoni, Luca ; Bigot, J.-Y. ; Schwalbach ; Follenius-Wund ; Pigault ; Bourotte

Author_Institution

IPCMS, Strasbourg, France

fYear

2002

fDate

19-24 May 2002

Firstpage

241

Lastpage

242

Abstract

Summary form only given. The green fluorescent protein is one of the most popular markers for in vivo labeling and single protein detection. We studied the dynamical properties of the emission of the GFPuv recombinant protein with the pump-probe technique on a purified sample in aqueous solution with femtosecond time resolution. Our comparative study of the excited states dynamics seems to exclude the presence of two distinct electronic states in the isolated chromophore and confirms the importance of the interaction between the chromophore and the proteic cage for a full understanding of the emission properties of the GFP.

Keywords

Raman spectra; biochemistry; bioluminescence; excited states; fluorescence; molecular biophysics; optical saturable absorption; photochemistry; proteins; time resolved spectra; aqueous solution; chromophore; differential transmission; electronic states; excited state bleaching; excited state population dynamics; femtosecond time resolution; fluorescence; green fluorescent protein; induced absorption; photophysics; proteic cage; pump-probe technique; recombinant protein; solvent Raman scattering; synthetic analogue; ultrafast gain dynamics; Bioluminescence; Fluorescence; Optical saturation; Proteins; Raman scattering;

fLanguage

English

Publisher

ieee

Conference_Titel

Quantum Electronics and Laser Science Conference, 2002. QELS '02. Technical Digest. Summaries of Papers Presented at the

Conference_Location

Long Beach, CA, USA

Print_ISBN

1-55752-708-3

Type

conf

DOI

10.1109/QELS.2002.1031369

Filename

1031369