Title :
High-throughput protein binding end-points and kinetics in microarrays using label-free OI-RD microscopes
Author :
Landry, J.P. ; Sun, Y.S. ; Fei, Y.Y. ; Lam, K.S. ; Zhu, X.D.
Author_Institution :
Dept. of Phys., Univ. of California at Davis, Davis, CA
Abstract :
The potential of biomolecular microarrays on glass for high-throughput kinetics assays has not previously been fully exploited. We demonstrate real-time label-free optical detection of antibodies binding to drug-antigen microarrays using oblique-incidence reflectivity difference (OI-RD) microscopes.
Keywords :
arrays; biochemistry; biological techniques; ellipsometry; molecular biophysics; optical microscopy; proteins; reflectivity; biomolecular microarrays; drug-antigen microarrays; high-throughput kinetics assays; high-throughput protein binding; label-free OI-RD microscopes; oblique-incidence reflectivity difference microscopes; polarization-modulation ellipsometry; real-time label-free optical antibodies detection; Biomedical optical imaging; Biosensors; Glass; High speed optical techniques; Kinetic theory; Optical films; Optical microscopy; Optical sensors; Proteins; Substrates; 170.1420; 170.1580; 180.5810; 240.2130;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
