Title :
Recent advances in image analysis of chromosomes subjected to HG-banding and fluorescent in situ hybridization (FISH)
Author :
Novak, Angelina ; Jovanovic, Aleksandar
Author_Institution :
Inst. of Hematology, Med. Center of Srbija, Serbia
Abstract :
The use of cooled charge-coupled device (CCD) cameras and recently developed GIS software offer new opportunities for the analysis of chromosomes subjected to HG-banding. We evaluated the efficiency and sensitivity of CCD microscopy to the detection of the genomic origin of marker chromosome with partial trisomy in cells from the bone marrow of the patient with Myelodysplastic syndrome (MDS-RA). The results of our fluorescent in situ hybridization (FISH) studies with DNA probes, performed for the identification of the chromosomal origin of the marker chromosome in metaphases, confirm previous findings. Ability of this procedure to integrate fluorescence signals has contributed to the increase in sensitivity of FISH in interphase cells
Keywords :
CCD image sensors; biology computing; cancer; cellular biophysics; fluorescence; genetics; image recognition; medical expert systems; medical image processing; optical microscopy; CCD cameras use; CCD microscopy; DNA probes; HG-banding; Myelodysplastic syndrome; automatic comparison; bone marrow cells; chromosomal origin; chromosomes; cytogenetics; efficiency; fluorescence signals; fluorescent in situ hybridization; genomic origin; group for intelligent systems software; image analysis; interphase cells; metaphases; partial trisomy; photometric curves; sensitivity; Bioinformatics; Biological cells; Charge coupled devices; Charge-coupled image sensors; Fluorescence; Genomics; Geographic Information Systems; Image analysis; Marine animals; Microscopy;
Conference_Titel :
Bioelectromagnetism, 1998. Proceedings of the 2nd International Conference on
Conference_Location :
Melbourne, Vic.
Print_ISBN :
0-7803-3867-7
DOI :
10.1109/ICBEM.1998.666409
