Title :
Dual-color superresolution imaging using genetically expressed probes
Author :
Shroff, Hari ; Galbraith, Catherine G. ; Galbraith, James A. ; White, Helen ; Gillette, Jennifer ; Olenych, Scott ; Davidson, Michael W. ; Betzig, Eric
Author_Institution :
Howard Hughes Med. Inst., Ashburn, VA
Abstract :
We report dual-color superresolution imaging using endogenously expressed fluorescent proteins. An imaging resolution of 20-30 nm facilitates study of the ultrastructural relationship between proteins present in adhesion complexes at the surfaces of whole, fixed cells.
Keywords :
adhesion; biological techniques; biomechanics; cellular biophysics; fluorescence; genetics; image resolution; molecular biophysics; proteins; adhesion complexes; biological cell surface; dual-color imaging; fluorescent proteins; genetically expressed probes; proteins; superresolution imaging; ultrastructural relationship; Biological information theory; Biomedical imaging; Fluorescence; High-resolution imaging; Image resolution; Labeling; Microscopy; Optical imaging; Probes; Proteins; (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
