Title :
Superresolution imaging in live bacterial cells by single-molecule active-control microscopy
Author :
Biteen, Julie S. ; Thompson, Michael A. ; Tselentis, Nicole K. ; Shapiro, Lucy ; Moerner, W.E.
Author_Institution :
Stanford Univ., Stanford, CA
Abstract :
Imaging of a nanoscale emitter naturally provides position information beyond the diffraction limit. We use active control to switch single fluorophores on and off to image structures with 40-nm resolution in a living cell.
Keywords :
biological techniques; cellular biophysics; image resolution; microorganisms; molecular biophysics; optical microscopy; live bacterial cell imaging; nanoscale emitter; single fluorophores; single-molecule active-control microscopy; superresolution imaging; Biological cells; Biomedical optical imaging; Cells (biology); Fluorescence; Image resolution; Microorganisms; Nanobioscience; Optical imaging; Optical microscopy; Proteins; (100.6640) Superresolution; (170.2520) Fluorescence microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
