Title :
Depletion dynamics for stimulated emission depletion (STED) microscopy
Author :
Chiang, Margaret C. ; Garcia, Juan C. ; Liu, Jia-Ming
Author_Institution :
Electr. Eng. Dept., Univ. of California, Los Angeles, CA
Abstract :
We present preliminary experimental data revealing depletion dynamics for stimulated emission depletion (STED) microscopy as a function of excitation intensity, depletion intensity, time delay, and fluorophore damage. FluoSpheres 350/440(invitrogen) is used.
Keywords :
biochemistry; biological techniques; fluorescence; laser beam applications; molecular biophysics; optical microscopy; stimulated emission; FluoSpheres 350; FluoSpheres 440; STED microscopy; biological field; depletion dynamics; depletion intensity; excitation intensity; far-field light microscopy technique; fluorophore damage; invitrogen; stimulated emission depletion microscopy; time delay; Biological information theory; Choppers; Delay effects; Filters; Fluorescence; Laser excitation; Lenses; Optical microscopy; Spatial resolution; Stimulated emission; (110.0180) Microscopy; (180.2520) Fluorescence microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
