Title :
FRET detection in the plasma membrane using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy
Author :
Blandin, P. ; Leveque-Fort, S. ; Lecart, S. ; Druon, F. ; Georges, P. ; Cossec, J.C. ; Potier, M.C. ; Lenkei, Z.
Author_Institution :
Lab. Charles Fabry de l´´Inst. d´´Opt., Univ. Paris-Sud, Palaiseau
Abstract :
We developed a total internal reflection fluorescence lifetime imaging microscope to perform functional imaging of living cells membranes labeled with FRET couples. Forster resonance energy transfer efficiency can thus be followed with subwavelength axial resolution.
Keywords :
biological techniques; biomembranes; cellular biophysics; fluorescence; laser beam applications; optical microscopy; radiative lifetimes; FRET detection; Forster resonance energy transfer; biological processes; fluorophores; functional imaging; living cell membrane; picosecond supercontinuum fibre laser; plasma membrane; subwavelength axial resolution; total internal reflection fluorescence lifetime imaging microscopy; Biomedical measurements; Biomedical optical imaging; Biomembranes; Fluorescence; Image resolution; Microscopy; Optical filters; Optical imaging; Optical reflection; Plasmas; (170.6920) Time resolved imaging; (180.2520) Fluorescence microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
