Probing bacterial surfaces using 4Pi spectral self-interference fluorescence microscopy

Author

Dogan, Mehmet ; Goldberg, Bennett B. ; Jain, Sumita ; Goldberg, Marcia B. ; Swan, Anna K. ; Unlu, M. Selim

Author_Institution

Dept. of Phys., Boston Univ., Boston, MA

fYear

2008

fDate

4-9 May 2008

Firstpage

1

Lastpage

2

Abstract

We present nanometer scale axial localization of fluorescent markers to probe subcellular structures using self-interference based fluorescence microscopy. We show probing the membrane topography of a gram-negative bacterium, Shigella flexneri, and discuss further applications.

Keywords

biomedical optical imaging; biomembranes; cellular biophysics; fluorescence; microorganisms; optical microscopy; 4Pi spectral self- interference fluorescence microscopy; Shigella flexneri; bacterial surfaces; fluorescent markers; gram-negative bacterium; membrane topography; nanometer scale axial localization; probe subcellular structures; self-interference; Biomedical imaging; Biomedical optical imaging; Biomembranes; Fluorescence; Interference; Microorganisms; Optical microscopy; Probes; Stimulated emission; Surface topography; (170.0170) Medical optics and biotechnology; (180.1790) Confocal microscopy; (180.2520)Fluorescence microscopy; (180.3170) Interference microscopy;

fLanguage

English

Publisher

iet

Conference_Titel

Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on

Conference_Location

San Jose, CA

Print_ISBN

978-1-55752-859-9

Type

conf

Filename

4571312