Free radical activity and enhancement of chemotherapy by low-level ultrasound

Author

Harrison, G.H. ; Balcer-Kubiczek, E.K.

Author_Institution

Dept. of Radiat. Oncology, Maryland Univ., Baltimore, MD

fYear

1993

fDate

31 Oct-3 Nov 1993

Firstpage

921

Abstract

The mechanism of enhancement of chemotherapy by low-level ultrasound (US) (1.62 MHz tone burst, 10% duty cycle, 0.25 W/cm², 0.28 MPa, and 2.0 MHz center frequency pulsed, 8-cycle wave form, 1 KHz prf, 0.18 W/cm², 1 MPa) was investigated with respect to free radical activity. The cytotoxicity of Adriamycin and diaziquone was enhanced by US; both these compounds by themselves act in part through free radical mechanisms. The compounds 5-fluorouracil and cisplatin, which do not exhibit free radical activity by themselves, were not potentiated by US. Electron spin resonance spin trapping measurements showed that US by itself caused hydroxyl (O˙H) radical production in water comparable to that of 0.5 Gy of X-rays, but had no appreciable effect on clonogenic survival of CHO cells. Added Adriamycin did not change the yield of hydroxyl radicals

Keywords

biomedical ultrasonics; paramagnetic resonance of free radicals; radiation therapy; ultrasonic effects; 1 kHz; 1.62 MHz; 2 MHz; 5-fluorouracil; 8-cycle wave form; Adriamycin; CHO cells; cancer therapy; chemotherapy enhancement; cisplatin; clonogenic survival; cytotoxicity; diaziquone; electron spin resonance spin trapping measurements; free radical activity; hydroxyl; low-level ultrasound; sonodynamic tumour therapy; Cancer; Electron traps; Frequency; Laboratories; Medical treatment; Oncology; Paramagnetic resonance; Production; Temperature; Ultrasonic imaging;

fLanguage

English

Publisher

ieee

Conference_Titel

Ultrasonics Symposium, 1993. Proceedings., IEEE 1993

Conference_Location

Baltimore, MD

Print_ISBN

0-7803-2012-3

Type

conf

DOI

10.1109/ULTSYM.1993.339657

Filename

339657