Title :
Multiplexed fluorescence lifetime microscopy by frequency-sweeping Fourier spectroscopy
Author :
Zhao, Ming ; Peng, Leilei
Author_Institution :
Coll. of Opt. Sci., Univ. of Arizona, Tucson, AZ, USA
Abstract :
We have developed a multiplexed fluorescence lifetime confocal scanning microscopy method based on frequency sweeping Fourier fluorescence lifetime spectroscopy. It simultaneously obtains nanosecond fluorescence lifetime images at multiple excitation wavelengths.
Keywords :
Fourier transform spectroscopy; biomedical optical imaging; fluorescence; nanobiotechnology; optical microscopy; frequency sweeping Fourier fluorescence lifetime spectroscopy; multiple excitation wavelengths; multiplexed fluorescence lifetime confocal scanning microscopy method; nanosecond fluorescence lifetime imaging; Fluorescence; Microscopy; Mirrors; Multiplexing; Optical imaging; Optical interferometry; Optical microscopy;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2011 Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4577-1223-4
