Title :
Numerical analysis of FRAP experiments for DNA replication and repair
Author :
Cinquemani, Eugenio ; Roukos, Vassilis ; Lygerou, Zoi ; Lygeros, John
Author_Institution :
Autom. Control Lab., ETH Zurich, Zurich, Switzerland
Abstract :
In this paper we describe a stochastic model for particle diffusion and binding within cell nuclei, and a numerical approximation of it that is well suited for numerical simulation and investigation of Fluorescence Recovery After Photobleaching (FRAP) experiments. An overview of the theory and of the implementation of the method is given. Simulation results are reported and are used to analyze data from real photobleaching experiments, mainly at a qualitative level.
Keywords :
DNA; biocomputing; biology computing; numerical analysis; DNA replication; cell nuclei; deoxyribonucleic acid; fluorescence recovery; numerical analysis; numerical approximation; numerical simulation; particle diffusion; photobleaching experiments; stochastic model; Bioinformatics; Biological system modeling; DNA; Data analysis; Fluorescence; Genomics; Numerical analysis; Numerical simulation; Photobleaching; Proteins; Biological systems; Monte Carlo methods; Numerical simulation; Parameter identification; Stochastic approximation;
Conference_Titel :
Decision and Control, 2008. CDC 2008. 47th IEEE Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-3123-6
Electronic_ISBN :
0191-2216
DOI :
10.1109/CDC.2008.4738718
