Title :
Effect of fluorescently labeling protein probes on kinetics of protein-ligand reactions
Author :
Sun, Y.S. ; Landry, J.P. ; Fei, Y.Y. ; Luo, J.T. ; Wang, X.B. ; Lam, K.S. ; Zhu, X.D.
Author_Institution :
Dept. of Phys., Univ. of California at Davis, Davis, CA
Abstract :
We study the kinetic effect of extrinsic fluorescent labeling agents on protein-ligand binding affinity and find that the kinetics is related to the loss or change of protein function when proteins are fluorescent-labeled.
Keywords :
biochemistry; fluorescence; molecular biophysics; proteins; fluorescent labeling; kinetic effect; protein probes; protein-ligand binding affinity; protein-ligand reactions; reaction kinetics; Biomedical optical imaging; Fluorescence; Fresnel reflection; Kinetic theory; Labeling; Microscopy; Optical surface waves; Peptides; Probes; Proteins; 170.0170; 180.0180; 240.0310; 310.0310;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
