The use of hemizona assay as a model for sperm-oocyte interaction in the evaluation of the optimal sperm preparation technique

Author

Paz, Gerardo ; Amit, A.

Author_Institution

Inst. for the Study of Fertility, Tel Aviv Univ.

fYear

1995

fDate

15-18 Feb 1995

Abstract

This study was designed to evaluate the effect of different sperm preparation methods on sperm binding capacity by using the hemizona assay (HZA). Seventy men were included in the study. Four different techniques (swim-up, TEST-yolk buffer [TYB], Percoll and pentoxifylline [PTX]) were used for preparation of sperm suspensions. The routine swim-up preparation was used as the reference method. Only TYB and PTX exhibited an overall statistically significant improvement in sperm binding capacity in comparison with the swim-up preparation method (P=0.01 and P=0.001, respectively)

Keywords

biological specimen preparation; cellular biophysics; Percoll; TEST-yolk buffer; hemizona assay; infertility clinic; optimal sperm preparation technique evaluation; pentoxifylline; sperm binding capacity; sperm suspensions; sperm-oocyte interaction model; swim-up; Hospitals; Humans; In vitro fertilization; Indium; Laboratories; Micromanipulators; Temperature; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1995 and 14th Conference of the Biomedical Engineering Society of India. An International Meeting, Proceedings of the First Regional Conference., IEEE

Conference_Location

New Delhi

Print_ISBN

0-7803-2711-X

Type

conf

DOI

10.1109/RCEMBS.1995.511696

Filename

511696