Integrating combinatorial synthesis on a single glass microchip

Summary form only given. Combinatorial chemistry is becoming prevalent methodology in preparing samples for high-throughput drug screening. Here we report a prototype of a combinatorial synthetic system integrated on a single glass microchip. By sandwiching a glass plate having pierced holes between two channel-etched glass surfaces, we made a glass chip in which channels in upper and bottom plates can cross without contacting each other (two-level crossing). To check the 3-D channel network, combinatorial amide syntheses by mixing two acid chlorides with two amines were carried out.