Characterization and bioanalytical application of innovative plasmonic nanostructures

Author

Cialla, D. ; Weber, K. ; Boehme, R. ; Huebner, U. ; Schneidewind, H. ; Zeisberger, M. ; Mattheis, R. ; Popp, J.

Author_Institution

Abbe Center of Photonics, Friedrich Schiller Univ. Jena, Jena, Germany

fYear

2011

fDate

13-16 Nov. 2011

Firstpage

1

Lastpage

8

Abstract

To combine fluorescence and surface enhanced Raman spectroscopy (SERS) utilizing the same plasmonic array, a silver deposited micro fabricated quartz grating prepared by electron beam lithography is used. The most efficient Raman signal amplification is achieved for molecules directly adsorbed on the metallic surface, while fluorescence is quenched under these conditions. By careful adjustment, fluorescence is also enhanced which is known as metal enhanced fluorescence. In this contribution, the combined fluorescence and SERS readout is verified by a DNA detection scheme based on the usage of the label molecule ATTO565.

Keywords

DNA; biological techniques; diffraction gratings; electron beam lithography; fluorescence; molecular biophysics; nanostructured materials; plasmonics; quartz; silver; surface enhanced Raman scattering; ATTO565; Ag; DNA detection; SERS; SiO₂; electron beam lithography; fluorescence; plasmonic nanostructures; quartz grating; quenching; surface enhanced Raman spectroscopy; Abstracts; Biomedical optical imaging; Nanostructures; Optical sensors; DNA detection scheme; SERS; electron beam lithography; fluorescence; nanostructured and regularly patterned plasmonic arrays;

fLanguage

English

Publisher

ieee

Conference_Titel

Communications and Photonics Conference and Exhibition, 2011. ACP. Asia

Conference_Location

Shanghai

ISSN

2162-108X

Print_ISBN

978-0-8194-8961-6

Type

conf

DOI

10.1117/12.903800

Filename

6210872