Title :
Rebinding dynamics of CO to Guanidine HC1-denatured heme proteins
Author :
Park, Jaeheung ; Kim, Jooyoung ; Lee, Taegon ; Lim, Manho
Author_Institution :
Dept. of Chem., Pusan Nat. Univ., Busan
Abstract :
Rebinding dynamics of CO photolyzed from GdnHCl-denatured HbCO and MbCO in D20 at 283K was probed by femtosecond mid-IR spectroscopy. The rebinding of CO to the denatured protein is much more efficient and faster than that to the native protein, suggesting that native structure is crucial for efficient expulsion of toxic CO.
Keywords :
carbon compounds; high-speed optical techniques; infrared spectra; molecular biophysics; proteins; toxicology; CO; Guanidine HCl-denatured heme proteins; femtosecond mid-IR spectroscopy; rebinding dynamics; temperature 283 K; toxic carbon monoxide expulsion; Broadband amplifiers; Optical amplifiers; Optical pulse generation; Optical pulses; Probes; Proteins; Pulse amplifiers; Semiconductor optical amplifiers; Spectroscopy; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics - Pacific Rim, 2007. CLEO/Pacific Rim 2007. Conference on
Conference_Location :
Seoul
Print_ISBN :
978-1-4244-1173-3
Electronic_ISBN :
978-1-4244-1174-0
DOI :
10.1109/CLEOPR.2007.4391605
