A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

Author

Joon-Ho Kim ; Byoung-Gyun Kim ; Hyukjun Nam ; Dae-Eun Park ; Kwang-Seok Yun ; Jun-Bo Yoon ; Jichang You ; Euisik Yoon

Author_Institution

Dept. of Electr. Eng. & Comput. Sci., Korea Adv. Inst. of Sci. & Technol., Taejon, South Korea

fYear

2002

fDate

24-24 Jan. 2002

Firstpage

133

Lastpage

136

Abstract

A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2 /spl mu/m diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynolds number below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15 ng/cm/sup 2/ and a minimum extractable input concentration of 100 copies/200 /spl mu/L. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

Keywords

DNA; biochemistry; biological specimen preparation; filtration; microfluidics; molecular biophysics; separation; 2.2 micron; DNA purification chip; binding capacity; disposable DNA sample preparation microfluidic chip; hole array; low Reynolds number mixing; low-cost disposable sample preparation; microfilter; micromixer; microsieve type filter; minimum extractable input concentration; nucleic acid probe assay; photosensitive glass; DNA; Filters; Microfiltration; Microfluidics; Nickel; Probes; Purification; Red blood cells; Resists; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2002. The Fifteenth IEEE International Conference on

Conference_Location

Las Vegas, NV, USA

ISSN

1084-6999

Print_ISBN

0-7803-7185-2

Type

conf

DOI

10.1109/MEMSYS.2002.984222

Filename

984222