Title :
Combining phase contrast microscopy and laser tweezers Raman spectroscopy to characterize germination of single bacterial spores
Author :
Kong, Lingbo ; Zhang, Pengfei ; Setlow, Peter ; Li, Yong-qing
Author_Institution :
Dept. of Phys., East Carolina Univ., Greenville, NC, USA
Abstract :
We report a method that combines external phase contrast microscopy, Raman spectroscopy and optical tweezers to monitor a variety of changes during the germination of single bacterial spores.
Keywords :
Raman spectroscopy; biological techniques; microorganisms; optical microscopy; radiation pressure; external phase contrast microscopy; laser tweezers Raman spectroscopy; single bacterial spore germination; Biomedical optical imaging; Microscopy; Optical imaging; Optical microscopy; Optical refraction; Optical scattering; Raman scattering;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2011 Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4577-1223-4
