Title :
In vivo two-photon imaging of cortical vasculature in mice to 1.5-mm depth with 1280-nm excitation
Author :
Kobat, Demirhan ; Horton, Nicholas G. ; Xu, Chris
Author_Institution :
Sch. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
Abstract :
Deep tissue multiphoton imaging of mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.5-mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.
Keywords :
bio-optics; biomedical optical imaging; blood vessels; brain; light scattering; two-photon spectroscopy; deep tissue multiphoton imaging; depth 1.5 mm; fundamental depth limit; in vivo two photon imaging; mouse brain; mouse cortex; murine cortical vasculature; scattering tissue; wavelength 1280 nm; Attenuation; Fluorescence; Imaging; In vivo; Mice; Scattering; Signal to noise ratio;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2011 Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4577-1223-4
