Title :
Biological cell assays on an electrokinetic microchip
Author :
Andersson, P.E. ; Li, P.C.H. ; Smith, R. ; Szarka, R.J. ; Harrison, D.J.
Author_Institution :
Dept. of Chem., Alberta Univ., Edmonton, Alta., Canada
Abstract :
A technique for single cell assay of non adherent cells is demonstrated by using microfabricated flow manifolds and video fluorescence microscopy. The kinetic cellular event of calcium flux release was determined in human lymphocytes. Transport, incubation and reactions on the microchip was accomplished using either electroosmotic or hydrodynamic pumping
Keywords :
bioelectric phenomena; biomembrane transport; biosensors; blood; calcium; electrokinetic effects; fluorescence; optical microscopy; Ca; biological cell assays; calcium flux release; electrokinetic microchip; electroosmotic pumping; human lymphocytes; hydrodynamic pumping; incubation; kinetic cellular event; microfabricated flow manifolds; nonadherent cells; single cell assay technique; video fluorescence microscopy; Biological cells; Calcium; Cells (biology); Electrokinetics; Fluorescence; Fungi; Humans; Kinetic theory; Microscopy; Red blood cells;
Conference_Titel :
Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
Conference_Location :
Chicago, IL
Print_ISBN :
0-7803-3829-4
DOI :
10.1109/SENSOR.1997.635477
