Miniaturized two-photon fluorescence and second harmonic generation microscope with a 24Hz frame-rate

Author

Liu, Tzu-Ming ; Chan, Ming-Che ; Chen, I-Hsiu ; Chia, Shih-Shuan ; Sun, Chi-Kuang

Author_Institution

Dept. of Electr. Eng., Nat. Taiwan Univ., Taipei

fYear

2008

fDate

4-9 May 2008

Firstpage

1

Lastpage

2

Abstract

With miniaturized tube lenses and two-dimensional asynchronous scanning of the microelectro-mechanical-system mirror, we demonstrated a 24 Hz frame-rate miniaturized two-photon fluorescence/second-harmonic-generation microscope system. Sub-micron transverse resolution of sectioning images can be achieved.

Keywords

bioMEMS; biomedical optical imaging; fluorescence; image resolution; micro-optomechanical devices; microlenses; micromirrors; optical harmonic generation; optical microscopy; two-photon processes; image sectioning; microelectro-mechanical-system mirror; miniaturized tube lenses; miniaturized two-photon fluorescence microscope; second harmonic generation microscope; sub-micron transverse resolution; two-dimensional asynchronous scanning; Fluorescence; Frequency conversion; Image resolution; Laser beams; Laser mode locking; Lenses; Micromechanical devices; Microscopy; Mirrors; Optical harmonic generation; (180.4315) Nonlinear microscopy; (190.4710) Optical nonlinearities in organic materials;

fLanguage

English

Publisher

iet

Conference_Titel

Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on

Conference_Location

San Jose, CA

Print_ISBN

978-1-55752-859-9

Type

conf

Filename

4572454