Second harmonic generation microscopy on the polyhedral inclusion bodies of nuclear polyhedrosis viruses

Author

Liu, Tzu-Ming ; Lee, Yen-Wei ; Chang, Chieh-Feng ; Yeh, Shih-Chia ; Wang, Chung-Hsiung ; Chu, Shi-Wei ; Sun, Chi-Kuang

Author_Institution

Grad. Inst. of Photonics & Optoelectron., Nat. Taiwan Univ., Taipei

fYear

2008

fDate

4-9 May 2008

Firstpage

1

Lastpage

2

Abstract

We demonstrate second-harmonic-generation (SHG) microscopic imaging on the polyhedral inclusion bodies (PIB) of nucleopolyhedrovirus in living cells. Due to a body-centeredcubic arrangement of polyhedrin trimers, these PIBs generate SHG with polarization anisotropy.

Keywords

biological techniques; cellular biophysics; light polarisation; microorganisms; optical harmonic generation; optical microscopy; body-centered-cubic arrangement; living cells; microscopic imaging; nuclear polyhedrosis viruses; nucleopolyhedrovirus; polarization anisotropy; polyhedral inclusion bodies; polyhedrin trimers; second harmonic generation; Biomedical optical imaging; Frequency conversion; Nonlinear optics; Optical harmonic generation; Optical microscopy; Optical polarization; Optical pulses; Photonics; Ultrafast optics; Viruses (medical); (180.4315) Nonlinear microscopy; (190.3970) Microparticle nonlinear optics; (190.4710) Optical nonlinearities in organic materials;

fLanguage

English

Publisher

iet

Conference_Titel

Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on

Conference_Location

San Jose, CA

Print_ISBN

978-1-55752-859-9

Type

conf

Filename

4572460