Title :
Design and characterization of a silver-enhanced gold nanoparticle-based biochip
Author :
Liu, Yang ; Zhang, Deng ; Alocilja, Evangelyn ; Chakrabartty, Shantanu
Author_Institution :
Dept. of Electr. & Comput. Eng., Michigan State Univ., East Lansing, MI, USA
Abstract :
Silver-enhanced labeling method that is employed in immunochromatographic assay provides an effective way of improving the sensitivity of detecting pathogens. In this paper, we apply the silver enhancement approach for providing signal amplification in conductimetric biochips which employ gold nanoparticles to bridge on a high-density microelectrode array for detecting gold nanoparticles. One of the measurement methods presented in this paper is the silver enhancing time that is shown to be an indicator of the concentration of gold nanoparticles. The method provides an alternative to existing detection methods and it is straightforward to achieve high-sensitivity in detection. The measured results presented in this paper are based on a high-density interdigital microelectrodes with a 4 mum gap where we show detection limits as low as 25 femtomolar.
Keywords :
bioMEMS; biosensors; chromatography; gold; lab-on-a-chip; microelectrodes; microorganisms; nanoparticles; silver; Ag; Au; MEMS technology; biosensor; conductimetric biochip signal amplification; distance 4 mum; gold nanoparticle; high-density interdigital microelectrode array; immunochromatographic assay; pathogen detection; silver-enhanced labeling method; Biomedical optical imaging; Biosensors; Gold; Immune system; Microelectrodes; Nanoparticles; Optical detectors; Optical sensors; Silver; Ultrafast optics; Gold nanoparticle; biochip; microelectrodes array; silver enhancement;
Conference_Titel :
Circuits and Systems, 2009. ISCAS 2009. IEEE International Symposium on
Conference_Location :
Taipei
Print_ISBN :
978-1-4244-3827-3
Electronic_ISBN :
978-1-4244-3828-0
DOI :
10.1109/ISCAS.2009.5118292