DocumentCode :
2267516
Title :
Hierarchical cluster analysis to detect coordinated protein expression in metabolically engineered Zymomonas mobilis
Author :
Hodge, David ; Karim, M. Nazmul ; Reardon, Kenneth F.
Author_Institution :
Dept. of Chem. Eng., Colorado State Univ., Fort Collins, CO, USA
Volume :
3
fYear :
2003
fDate :
4-6 June 2003
Firstpage :
2081
Abstract :
Two dimensional gel electrophoresis (2-DE) was used to profile protein expression patterns in a metabolically engineered Zymomonas mobilis strain. An application of the hierarchical cluster analysis to dynamic protein expression patterns determined that functionally related proteins show similar expression trajectories. The three functionally related classes of proteins include: Entner-Douderoff (ED) and fermentative pathway enzymes, recombinant pentose phosphate (PP) pathway enzymes, and stress proteins. Findings included identifying a cellular stress response as a result of the utilization of the foreign metabolic pathway.
Keywords :
biotechnology; electrophoresis; enzymes; fermentation; microorganisms; proteins; 2D gel electrophoresis; Entner-Douderoff enzymes; cellular stress; fermentative pathway enzymes; foreign metabolic pathway; hierarchical cluster analysis; metabolic engineering; protein expression patterns; recombinant pentose phosphate pathway enzymes; stress proteins; zymomonas mobilis strain; Biochemistry; Bioinformatics; Genomics; Microorganisms; Power engineering and energy; Protein engineering; Proteomics; Renewable energy resources; Stress; Sugar;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
American Control Conference, 2003. Proceedings of the 2003
ISSN :
0743-1619
Print_ISBN :
0-7803-7896-2
Type :
conf
DOI :
10.1109/ACC.2003.1243380
Filename :
1243380
Link To Document :
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