Title :
Spectrally resolved TCSPC FRET imaging
Author :
Becker, W. ; Bergmann, A. ; Benndorf, K. ; Biskup, C. ; Zimmer, T.
Author_Institution :
Becker & Hickl GmbH, Berlin, Germany
Abstract :
Summary from only given. A new TCSPC imaging technique in conjunction with a laser scanning microscope and a multi-channel PMT yields time- and wavelength resolved fluorescence images. Applied to FRET in living cells, the technique delivers the decay components of the donor and acceptor fluorescence in all pixels of the image. The data can be used to build up images showing the amount of FRET in the individual parts of the cell.
Keywords :
biological techniques; cellular biophysics; fluorescence; imaging; photon counting; HEK cell; acceptor fluorescence; build up images; decay components; donor fluorescence; laser scanning microscope; living cells; multi-channel PMT; spectrally resolved time-correlated single photon counting FRET imaging; time-resolved fluorescence images; wavelength resolved fluorescence images; Bones; Breast tissue; Fluorescence; Image resolution; Muscles; Optical imaging; Polarization; Pulse measurements; Ultrafast optics; Vectors;
Conference_Titel :
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Long Beach, CA, USA
Print_ISBN :
1-55752-706-7
DOI :
10.1109/CLEO.2002.1034096
