• DocumentCode
    2269919
  • Title

    Nanometer X-ray microscopy of labeled live biological organisms with a nanosecond laser-plasma source

  • Author

    Al-Ani, M.R. ; Biggerstaff, J. ; Trujillo, M. ; Richardson, M.

  • Author_Institution
    Sch. of Opt., Central Florida Univ., Orlando, FL, USA
  • fYear
    2002
  • fDate
    24-24 May 2002
  • Firstpage
    370
  • Abstract
    Summary from only given. We demonstrate, the use of nanoparticle Au-labeling techniques in XRM using single, nanosecond, laser-plasma X-ray illumination of the specimen. In this case the specimen is in its natural state, and a high-resolution (-30 nm) X-ray radiograph is obtained in a time shorter than that required for any X-ray induced structural changes to the organism to be manifested on the image. Moreover we demonstrate, a technique in which combined labeled images of specific sites in the organism are obtained through both XRM and LSCM.
  • Keywords
    X-ray microscopy; biological specimen preparation; biological techniques; gold; plasma production by laser; radiography; Au; X-ray induced structural changes; high-resolution X-ray radiograph; labeled images; labeled live biological organisms; nanometer X-ray microscopy; nanoparticle Au-labeling techniques; nanosecond laser-plasma source; single nanosecond laser-plasma X-ray illumination; specific sites; Biological systems; Fluorescence; Microscopy; Nanobioscience; Optical imaging; Organisms; Pulsed laser deposition; Spatial resolution; X-ray imaging; X-ray lasers;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
  • Conference_Location
    Long Beach, CA, USA
  • Print_ISBN
    1-55752-706-7
  • Type

    conf

  • DOI
    10.1109/CLEO.2002.1034097
  • Filename
    1034097