DocumentCode :
2269919
Title :
Nanometer X-ray microscopy of labeled live biological organisms with a nanosecond laser-plasma source
Author :
Al-Ani, M.R. ; Biggerstaff, J. ; Trujillo, M. ; Richardson, M.
Author_Institution :
Sch. of Opt., Central Florida Univ., Orlando, FL, USA
fYear :
2002
fDate :
24-24 May 2002
Firstpage :
370
Abstract :
Summary from only given. We demonstrate, the use of nanoparticle Au-labeling techniques in XRM using single, nanosecond, laser-plasma X-ray illumination of the specimen. In this case the specimen is in its natural state, and a high-resolution (-30 nm) X-ray radiograph is obtained in a time shorter than that required for any X-ray induced structural changes to the organism to be manifested on the image. Moreover we demonstrate, a technique in which combined labeled images of specific sites in the organism are obtained through both XRM and LSCM.
Keywords :
X-ray microscopy; biological specimen preparation; biological techniques; gold; plasma production by laser; radiography; Au; X-ray induced structural changes; high-resolution X-ray radiograph; labeled images; labeled live biological organisms; nanometer X-ray microscopy; nanoparticle Au-labeling techniques; nanosecond laser-plasma source; single nanosecond laser-plasma X-ray illumination; specific sites; Biological systems; Fluorescence; Microscopy; Nanobioscience; Optical imaging; Organisms; Pulsed laser deposition; Spatial resolution; X-ray imaging; X-ray lasers;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Long Beach, CA, USA
Print_ISBN :
1-55752-706-7
Type :
conf
DOI :
10.1109/CLEO.2002.1034097
Filename :
1034097
Link To Document :
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