Title :
Zero-mode waveguides for single molecule analysis and fast DNA sequencing
Author :
Levene, M. ; Korlach, J. ; Turner, S. ; Craighead, H.G. ; Webb, W.W.
Author_Institution :
Dept. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
Abstract :
Summary form only given. Zero-mode waveguides enable the observation of which fluorescent nucleotide analog is being added to the double stranded product in the active site of the enzyme while excluding signal from freely diffusing fluorescent species. The effectiveness of zero-mode waveguides is demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside zero-mode waveguides, using micromolar concentrations of fluorescently labeled nucleotide analogs.
Keywords :
biological techniques; fluorescence; genetics; molecular biophysics; optical waveguides; proteins; DNA polymerase; active site; double stranded product; enzyme; fast DNA sequencing; fluorescently labeled nucleotide analogs; freely diffusing fluorescent species; micromolar concentrations; real-time observation; single molecule analysis; zero-mode waveguides; Biochemistry; Finite element methods; Fluorescence; Genomics; Polymers; Spectroscopy; Waveguide theory;
Conference_Titel :
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Long Beach, CA, USA
Print_ISBN :
1-55752-706-7
DOI :
10.1109/CLEO.2002.1034137
