DocumentCode
2270765
Title
Zero-mode waveguides for single molecule analysis and fast DNA sequencing
Author
Levene, M. ; Korlach, J. ; Turner, S. ; Craighead, H.G. ; Webb, W.W.
Author_Institution
Dept. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
fYear
2002
fDate
24-24 May 2002
Abstract
Summary form only given. Zero-mode waveguides enable the observation of which fluorescent nucleotide analog is being added to the double stranded product in the active site of the enzyme while excluding signal from freely diffusing fluorescent species. The effectiveness of zero-mode waveguides is demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside zero-mode waveguides, using micromolar concentrations of fluorescently labeled nucleotide analogs.
Keywords
biological techniques; fluorescence; genetics; molecular biophysics; optical waveguides; proteins; DNA polymerase; active site; double stranded product; enzyme; fast DNA sequencing; fluorescently labeled nucleotide analogs; freely diffusing fluorescent species; micromolar concentrations; real-time observation; single molecule analysis; zero-mode waveguides; Biochemistry; Finite element methods; Fluorescence; Genomics; Polymers; Spectroscopy; Waveguide theory;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
Conference_Location
Long Beach, CA, USA
Print_ISBN
1-55752-706-7
Type
conf
DOI
10.1109/CLEO.2002.1034137
Filename
1034137
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