• DocumentCode
    2276388
  • Title

    SRS microscopy as a low-invasive 3D bioimaging technique

  • Author

    Itoh, Kazuyoshi ; Ozeki, Yasuyuki

  • Author_Institution
    Grad. Sch., Dept. of Mater. & Life Sci., Osaka Univ., Suita, Japan
  • fYear
    2010
  • fDate
    12-16 July 2010
  • Firstpage
    1
  • Lastpage
    3
  • Abstract
    We introduce stimulated Raman scattering microscopy which allows us to visualize unstained live cells with high sensitivity and high contrast. The shot-noise limited sensitivity can be achieved based on high-frequency lock-in detection using subharmonically synchronized two-color pulses.
  • Keywords
    Raman spectroscopy; biomedical optical imaging; cellular biophysics; optical microscopy; shot noise; high-frequency lock-in detection; image contrast; lowinvasive 3D bioimaging technique; shot-noise limited sensitivity; stimulated Eaman scattering microscopy; subharmonically synchronized two-color pulses; unstained live cells; Laser noise; Microscopy; Optical microscopy; Raman scattering; Sensitivity; Synchronization;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Information Optics (WIO), 2010 9th Euro-American Workshop on
  • Conference_Location
    Helsinki
  • Print_ISBN
    978-1-4244-8226-9
  • Electronic_ISBN
    978-1-4244-8227-6
  • Type

    conf

  • DOI
    10.1109/WIO.2010.5582519
  • Filename
    5582519