Title :
Pulsed THz protein spectroscopy in organic solvents
Author :
Mickan, S.P. ; Dordick, J.S. ; Munch, J. ; Abbott, D. ; Zhang, X.-C.
Abstract :
Summary form only given. We are interested in using pulsed THz spectroscopy to characterize the conformation and hydration of enzymatic proteins for biochip analysis. We present measurements of the refractive index and extinction coefficient of organic solvents commonly used in studying protein activity. Our spectroscopic instrument is a pulsed THz system, based on a 100-fs Ti:sapphire laser, surface current generation and electro-optic detection. Preliminary results for iso-octane, toluene and cyclohexane show that the protein refractive index is profoundly affected by even small amounts of water present in the solvents. We present a detailed study of the hydration of protein in the non-polar hydrophilic solvent 1,4-dioxane.
Keywords :
biological techniques; extinction coefficients; infrared spectroscopy; molecular configurations; organic compounds; proteins; refractive index; solvation; solvent effects; 0.2 to 2 THz; 1,4-dioxane; 100 fs; 100-fs Ti:sapphire laser; biochip analysis; cyclohexane; electro-optic detection; enzymatic protein conformation; enzymatic protein hydration; extinction coefficient; iso-octane; nonpolar hydrophilic solvent; organic solvents; protein activity; pulsed THz protein spectroscopy; refractive index; surface current generation; toluene; Extinction coefficients; Optical pulse generation; Optical refraction; Optical variables control; Physics; Protein engineering; Refractive index; Solvents; Spectroscopy; Vents;
Conference_Titel :
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Long Beach, CA, USA
Print_ISBN :
1-55752-706-7
DOI :
10.1109/CLEO.2002.1034422
