Title :
An experimental study on protein-protein interaction using atomic force microscopy
Author :
Li, Mi ; Liu, Lianqing ; Xi, Ning ; Wang, Yuechao ; Dong, Zaili ; Li, Guangyong ; Xiao, Xiubin ; Zhang, Weijing
Author_Institution :
State Key Lab. of Robot., Chinese Acad. of Sci., Shenyang, China
Abstract :
Elucidating the underlying mechanism of the cellular physiological activities at the molecular level is currently an important research topic in life sciences. The invention of the atomic force microscopy (AFM) provided new methods for acquiring new cognition about cellular chemical processes. With the use of AFM, the specific recognition interaction between CD20 antigen and rituximab antibody on B lymphoma cells was explored under near-physiological conditions. The high quality AFM images of B lymphoma cells were acquired and the CD20-rituximab complex binding force was measured by AFM single-molecule force spectroscopy (SMFS). The experimental results provide new insights for understanding the CD20-rituximab specific interaction and lay the foundation for the further investigation of the mechanism of rituximab.
Keywords :
atomic force microscopy; cellular biophysics; proteins; scanning electron microscopy; B lymphoma cells; CD20 antigen; CD20-rituximab complex binding force; SEM; atomic force microscopy; cellular chemical processes; cellular physiological activities; protein-protein interaction; rituximab antibody; scanning electron microscopy; single-molecule force spectroscopy;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2010 10th IEEE Conference on
Conference_Location :
Seoul
Print_ISBN :
978-1-4244-7033-4
Electronic_ISBN :
1944-9399
DOI :
10.1109/NANO.2010.5697849
