Selective photoimmobilization of actin filaments for developing an intelligent nanodevice

In the present study we present a method of selective immobilization of actin filaments based on the bioaffinity reaction of streptavidin/biotion on an aminopropyltriethoxysilane (APTES)-functionalized glass surfaces. Atomic force microscopy was employed to verify APTES film with higher roughness compared with bare glass. Patterning was achieved since photobiotin was covalently attached to the surface with masks of varying size and geometry under ultraviolent light. The patterning was characterized by the fluorescence of dye-tagged streptavidin. After blocked, dye-tagged biotinylated actin patterning was obtained, implying the feasibility of a biomolecular transportation system based upon the selective immobilization of these motor proteins.