Title :
Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip
Author :
Charwat, V. ; Muellner, P. ; Hainberger, R. ; Purtscher, M. ; Ertl, P. ; Tedde, S. ; Hayden, O.
Author_Institution :
Health & Environ. Dept., AIT Austrian Inst. of Technol. GmbH, Vienna, Austria
Abstract :
We have developed a μ-fluidic biochip capable of monitoring in real-time cellular phenotype dynamics by non-invasively assessing cell viability and reproduction over long periods of time. The μ-fluidic biochip contains integrated organic photodiodes that continuously perform light scattering measurements of adherent cell populations. In the presented work, we experimentally show that light scattering measurements are an effective label-free method for accurately assessing cell number variations. Furthermore, results obtained from 3D FDTD simulations indicate that side scatter characteristics of cells allow in principle predictions on cellular health status.
Keywords :
bio-optics; cellular biophysics; finite difference time-domain analysis; lab-on-a-chip; light scattering; 3D FDTD simulation; adherent cell culture; cell viability; integrated organic photodiode; lab-on-a-chip; light scattering characteristic; light scattering measurement; microfluidic biochip capable; noninvasive assessment; real time cellular phenotype dynamics; Finite difference methods; Light scattering; Monitoring; Optical filters; Solid modeling; Three dimensional displays;
Conference_Titel :
Information Photonics (IP), 2011 ICO International Conference on
Conference_Location :
Ottawa, ON
Print_ISBN :
978-1-61284-315-5
DOI :
10.1109/ICO-IP.2011.5953790
