Long period grating-based fiber optic sensor for label-free DNA detection

Author

Sozzi, Michele ; Coscelli, Enrico ; Poli, Federica ; Cucinotta, Annamaria ; Selleri, Stefano ; Corradini, Roberto ; Marchelli, Rosangela ; Konstantaki, Mari ; Pissadakis, Stavros

Author_Institution

Inf. Eng. Dept., Univ. of Parma, Parma, Italy

fYear

2011

fDate

8-10 June 2011

Firstpage

1

Lastpage

3

Abstract

The feasibility of a DNA biosensor based on a long period grating inscribed in an optical fiber has been investigated. The sensor can perform a label-free detection, due to the functionalization of the optical fiber external surface with peptide nucleic acid probes matched with the DNA target strands. A wavelength shift of 1.2 nm for a 120 nM DNA solution has been measured. Fluorescence measurements have been also taken, in order to give a further proof of the fiber functionalization and hybridization.

Keywords

Bragg gratings; DNA; biosensors; fibre optic sensors; fluorescence; molecular biophysics; DNA biosensor; DNA solution; DNA target strands; fluorescence measurements; label-free DNA detection; long period grating-based fiber optic sensor; peptide nucleic acid probes; wavelength shift; Biomedical optical imaging; DNA; Fiber optics; Optical fiber sensors; Optical refraction; Optical variables control;

fLanguage

English

Publisher

ieee

Conference_Titel

BioPhotonics, 2011 International Workshop on

Conference_Location

Parma

Print_ISBN

978-1-4244-9836-9

Electronic_ISBN

978-1-4244-9835-2

Type

conf

DOI

10.1109/IWBP.2011.5954847

Filename

5954847