Accelerated differentiation of myoblast with electric pulses in vitro

An effect of repetitive electric pulses on differentiation of myoblasts has been studied in vitro. C2C12 (Mouse myoblast cell line originated with cross-striated muscle of C3H mouse) was exposed to repetitive electric pulses. The adhered cells were exposed to the electric field between two electrodes made of a platinum wire dipped in the medium at 37 degrees Celsius. The repetitive electric pulses at a period of one second were generated with a function generator. Variation was made on the amplitude of the pulse. To find the limit of amplitude of the electric pulse, the number of adhered cells was counted after exposure to the continuous electric stimulation for 72 hours. The cells were microscopically observed during cultivation, after electric stimulation with repetitive pulses for 72 hours. The responsive movement of myoblast was observed with the electric stimulation to confirm differentiation. The results show that the repetitive electric pulses accelerate differentiation of myoblast.