Accelerated differentiation of myoblast with high gravitational force in vitro

The accelerated differentiation of myoblast with high gravitational force has been studied in an experimental system with centrifugal force in vitro. Mouse myoblasts were seeded on a culture dish of 35 mm diameter, and cultured in the Dulbecco´s Modified Eagle´s Medium until the sub-confluent condition. To apply the excess gravitational force on the cultured cells, the dish was set in a conventional centrifugal machine. Constant gravitational force was applied to the cultured cells for three hours. Variation was made on the gravitational force (<;800 G) with control of the rotational speed of the rotator in the centrifugal machine. Morphology of the cells was observed with a phase-contrast microscope for eight days. The experimental results show that the myoblast thickens day by day after the exposure to the high gravitational force field.