Title :
Tissue response during staining and illumination of voltage-sensitive dye in rabbit myocardium
Author :
Nováková, M. ; Nogová, K. ; Bardonová, J. ; Provazník, I.
Author_Institution :
Dept. of Physiol., Masaryk Univ. Brno, Brno
fDate :
Sept. 30 2007-Oct. 3 2007
Abstract :
Voltage-sensitive dyes (VSDpsilas) undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and are therefore used for recording of monophasic action potentials (MAPs) by optical method. S.-c. phototoxic effects of VSDs appear under intense illumination and include alterations in heart electrical signals. To examine putative side effects of VSD di-4-ANEPPS in rabbit hearts, we studied electrogram, mean coronary flow and formation of hydroxyl radicals in the control, staining and washout periods and during illumination. Possible ultrastructural changes were examined by electron microscopy. Rabbit myocardium is resistant to di-4-ANEPPS side effects during staining and also resistant to bleaching during intense illumination since only minor changes in ECG and no change in other parameters were observed.
Keywords :
bioelectric potentials; biological tissues; biomedical measurement; dyes; electrocardiography; electron microscopy; fluorescence; haemodynamics; optical saturable absorption; VSD di-4-ANEPPS side effects; bleaching; di-4-amino-naphthyl-ethenyl-pyridinium; electrocardiography; electrogram; electron microscopy; fluorescence spectra; hydroxyl radicals formation; mean coronary flow; monophasic action potentials; rabbit hearts; rabbit myocardium; staining period; tissue response; voltage-sensitive dyes; washout period; Bleaching; Electron microscopy; Fluorescence; Heart; Lighting; Myocardium; Optical recording; Rabbits; Resistance; Voltage;
Conference_Titel :
Computers in Cardiology, 2007
Conference_Location :
Durham, NC
Print_ISBN :
978-1-4244-2533-4
Electronic_ISBN :
0276-6547
DOI :
10.1109/CIC.2007.4745573
