Title :
Magnetic particle enhanced microcantilever biosensor for rapid and Sensitive E. Coli detection
Author :
Polyzoev, Vasco ; Enikov, Eniko ; Heinze, Brian ; Yoon, Jeong-Yeol
Author_Institution :
Aerosp. & Mech. Eng. Dept., Univ. of Arizona, Tucson, AZ, USA
Abstract :
This paper presents a novel concept for integrating a magnetic microparticle sandwich assay onto a microcantilever beam, towards rapid and sensitive detection of E. Coli. The gold coated surface of the microcantilever beam and the surface of superparamagnetic microparticles are functionalized with anti-E. coli IgG so that they bind only in presence of and via the E. coli. An external magnetic field produced by an electromagnet is used to enhance the microcantilever deflection, and an optical lever method is used to sensitively detect it.
Keywords :
bioMEMS; biosensors; cantilevers; magnetic particles; microorganisms; superparamagnetism; 6magnetic particle enhanced microcantilever biosensor; anti-E. coli IgG; anti-E. coli immunoglobulin G; external magnetic field; magnetic microparticle sandwich assay; microcantilever beam gold coated surface; microcantilever deflection; optical lever method; rapid E. Coli detection; sensitive E. Coli detection; superparamagnetic microparticle surface; Biomedical optical imaging; Biosensors; Gold; Magnetic fields; Magnetic particles; Magnetic sensors; Micromagnetics; Optical sensors; Pathogens; Stress; E. coli detection; Magnetic microparticles; Microcantilever sensor; Optical lever;
Conference_Titel :
Optomechatronic Technologies, 2009. ISOT 2009. International Symposium on
Conference_Location :
Istanbul
Print_ISBN :
978-1-4244-4209-6
Electronic_ISBN :
978-1-4244-4210-2
DOI :
10.1109/ISOT.2009.5326138
