Recording I–V signals from INS-1 Cells using silicon based patch clamp device

Here we utilize a silicon based lateral patch clamp chip to study insulin secreting rat insulinoma (INS-1) cells. Electrical properties of various potassium and calcium ion channels were characterized. High gigaohm (GO) seals were achieved between the microfabricated glass capillary apertures and INS-1 cells. Longevity of the whole cell mode could be maintained for 1 hr. Steady state I-V plots elicited characteristic ion channel properties, and pave the way for future drug discovery experiments and developing a cheap platform using the silicon based approach.