Optimization of Extraction Process of Polysaccharides from Wild Termitomyces Albuminosus Using Response Surface Methodology

Polysaccharides from wild Termitomyces albuminosus (PWTA) were extracted using hot water extraction method at various extraction conditions. Single-factor experiments, an orthogonal L₉(3)⁴ test, and response surface methodology (RSM) were combined for experimental design and analysis, aiming to evaluate the effects of the extraction parameters on the extraction yield of PWTA and to obtain the optimal extraction conditions. The extraction temperature, extraction time, and powder to water ratio were found to have a significant influence on the extraction yield of PWTA. The extraction temperature was the most significant variable affecting the extraction yield of PWTA. The optimum conditions were: extraction temperature 95.9°C, extraction time 3.02 h, and powder to water ratio at 1:33.4. Under these conditions, the experimental extraction yield of PWTA was 7.25%, which is well matched with the predictive extraction yield by the RSM model, indicating that the RSM model was adequate for the extraction process.