Analysis of ultramicro biomedical samples with microfabricated chemical sensors: sample/reagent adsorption on alumina vs. pyrex substrates

Author

Hui, Kwong Yue ; Diefes, Richard S. ; Dudik, Laurie ; Liu, Chung-Chiun ; Gratzl, Miklós

Author_Institution

Dept. of Biomed. Eng., Case Western Reserve Univ., Cleveland, OH, USA

fYear

1994

fDate

1994

Firstpage

824

Abstract

Microfabricated platinum redox sensors can be used for indication in redox diffusional microtitration of microliter size body fluid samples. The sensor can be deposited onto different substrate materials. Adsorption of reagent and/or sample on alumina ceramic substrate versus Pyrex glass substrate is compared. It is demonstrated that in a classical iodimetric redox titration system, significant adsorption occurs on alumina ceramic substrate, causing irreproducible results. Reproducibility with a 1.7% coefficient of variation (CV) is achieved using a sensor deposited on Pyrex substrate for the analysis of 20 μL sample droplets

Keywords

adsorption; biomedical equipment; biosensors; chemical sensors; Al₂O₃; Pt; Pyrex glass substrate; alumina ceramic substrate; alumina substrates; classical iodimetric redox titration; coefficient of variation; microfabricated chemical sensors; microfabricated platinum redox sensors; microliter size body fluid samples; pyrex substrates; reagent adsorption; redox diffusional microtitration; reproducibility; sample adsorption; ultramicro biomedical samples; Amino acids; Amperometric sensors; Biochemistry; Biomedical measurements; Biosensors; Ceramics; Glass; Platinum; Reproducibility of results; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE

Conference_Location

Baltimore, MD

Print_ISBN

0-7803-2050-6

Type

conf

DOI

10.1109/IEMBS.1994.415163

Filename

415163