Title :
Analysis of ultramicro biomedical samples with microfabricated chemical sensors: sample/reagent adsorption on alumina vs. pyrex substrates
Author :
Hui, Kwong Yue ; Diefes, Richard S. ; Dudik, Laurie ; Liu, Chung-Chiun ; Gratzl, Miklós
Author_Institution :
Dept. of Biomed. Eng., Case Western Reserve Univ., Cleveland, OH, USA
Abstract :
Microfabricated platinum redox sensors can be used for indication in redox diffusional microtitration of microliter size body fluid samples. The sensor can be deposited onto different substrate materials. Adsorption of reagent and/or sample on alumina ceramic substrate versus Pyrex glass substrate is compared. It is demonstrated that in a classical iodimetric redox titration system, significant adsorption occurs on alumina ceramic substrate, causing irreproducible results. Reproducibility with a 1.7% coefficient of variation (CV) is achieved using a sensor deposited on Pyrex substrate for the analysis of 20 μL sample droplets
Keywords :
adsorption; biomedical equipment; biosensors; chemical sensors; Al2O3; Pt; Pyrex glass substrate; alumina ceramic substrate; alumina substrates; classical iodimetric redox titration; coefficient of variation; microfabricated chemical sensors; microfabricated platinum redox sensors; microliter size body fluid samples; pyrex substrates; reagent adsorption; redox diffusional microtitration; reproducibility; sample adsorption; ultramicro biomedical samples; Amino acids; Amperometric sensors; Biochemistry; Biomedical measurements; Biosensors; Ceramics; Glass; Platinum; Reproducibility of results; Substrates;
Conference_Titel :
Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE
Conference_Location :
Baltimore, MD
Print_ISBN :
0-7803-2050-6
DOI :
10.1109/IEMBS.1994.415163
