Effect of magnetic fields on the enzymatic activity of plasmin

Author

Iwasaka, Masakazu ; Ueno, Shoogo ; Tsuda, Hiroko

Author_Institution

Dept. of Comput. Sci. & Commun. Eng., Kyushu Univ., Fukuoka, Japan

fYear

1994

fDate

1994

Firstpage

762

Abstract

The authors investigated the effect of magnetic fields on the enzymatic activity of plasmin concerning the dissolution of thrombus. Plasmin activity in magnetic fields up to 8 T was assessed using a synthetic substrate for plasmin, D-Val-Leu-Lys-pNA. After 40 min or more incubation, absorbance of the mixture exposed to an 8 T magnetic field at 37°C was 7-11% lower than control sample. The results show that the enzymatic activity of plasmin was affected by a magnetic field at 8 T. This suggests that magnetic fields have an effect on the active center of the enzymatic reaction of plasmin with a substrate, especially on the hydrogen bonds of the charge-relay system

Keywords

biological effects of fields; biomagnetism; biomolecular effects of radiation; magnetic field effects; proteins; 37 C; 40 min; 8 T; D-Val-Leu-Lys-pNA; active center; charge-relay system; enzymatic reaction; hydrogen bonds; plasmin enzymatic activity; synthetic substrate; thrombus dissolution; Amino acids; Biomedical engineering; Computer science; Degradation; Hospitals; Hydrogen; Laboratories; Magnetic field measurement; Magnetic fields; Superconducting magnets;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE

Conference_Location

Baltimore, MD

Print_ISBN

0-7803-2050-6

Type

conf

DOI

10.1109/IEMBS.1994.415280

Filename

415280