Molecular cloning of ERG 2 gene to effect knockdown in chaetomium cupreum

Author

Odeph, Margaret ; Yang, Qian ; Phiri, Jackson

Author_Institution

Dept. of Life Sci. & Eng., Harbin Inst. of Technol., Harbin, China

fYear

2012

fDate

23-25 March 2012

Firstpage

25

Lastpage

28

Abstract

RNA interference has unveiled the hidden sound of silence in many organisms today. To follow suit, an intron-containing hairpin RNA (ihpRNA) plasmid vector, Psilent -1, with inverted repeats of size ~800bp was constructed. Rapid amplification of complementary DNA ends (RACE) revealed the 3´-full length of ERG 2 gene coding region as 989bp. This encodes 321 amino acids of molecular weight 33.89kDa and a theoretical isoelectric point of 5.05. This silencing construct is the underlying bedrock upon which work in the near future concerning ergosterol biosynthetic pathway and expression in C. cupreum at molecular level will richly overlay.

Keywords

DNA; RNA; cellular biophysics; genetics; microorganisms; molecular biophysics; molecular weight; 321 amino acids; Chaetomium cupreum; ERG 2 gene coding region; Psilent-1; RNA interference; complementary DNA ends; ergosterol biosynthetic pathway; intron-containing hairpin RNA plasmid vector; isoelectric point; molecular cloning; molecular level; molecular weight; Biochemistry; Bioinformatics; DNA; Fungi; Interference; RNA; Vectors;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Science and Technology (ICIST), 2012 International Conference on

Conference_Location

Hubei

Print_ISBN

978-1-4577-0343-0

Type

conf

DOI

10.1109/ICIST.2012.6221602

Filename

6221602