Title :
Multiphoton autofluorescence microscopy and second harmonic generation microscopy of oral epithelial neoplasms
Author :
Vargas, Gracie ; Shilagard, Tuya ; Ho, Ki-Hong ; McCammon, Susan
Author_Institution :
Univ. of Texas Med. Branch, Galveston, TX, USA
Abstract :
The objective of this study was to evaluate the nonlinear optical microscopy techniques of multiphoton autofluorescence microscopy (MPAM) and second harmonic generation microscopy (SHGM) for noninvasive imaging of oral epithelial carcinogenesis. In vivo imaging was performed in a hamster model for oral carcinogenesis to characterize optical and morphometric alterations during neoplastic transformation. Data is presented showing alterations in morphometry and collagen density during the precancerous phase of neoplastic transformation.
Keywords :
bio-optics; biomedical optical imaging; cancer; fluorescence; multiphoton processes; optical harmonic generation; optical microscopy; proteins; tumours; collagen density; morphometric alterations; morphometry; multiphoton autofluorescence microscopy; neoplastic transformation; noninvasive imaging; nonlinear optical microscopy technique; optical alterations; oral epithelial carcinogenesis; oral epithelial neoplasms; second harmonic generation microscopy; Animals; Cricetinae; Equipment Design; Equipment Failure Analysis; Mesocricetus; Mouth Neoplasms; Neoplasms, Glandular and Epithelial; Reproducibility of Results; Sensitivity and Specificity;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5332783
