Interrelationship among breakdown voltage, latency time and membrane conductance during electroporation of artificial lipid membranes

Author

Sharma, Vinod ; Tung, Leslie ; Murphy, John C.

Author_Institution

Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA

fYear

1994

fDate

1994

Firstpage

1177

Abstract

Electroporation of artificial lipid membranes was studied by applying a 10 μs, 300-600 mV rectangular voltage clamp pulse followed by a ramp of negative slope which decayed to zero in the subsequent 500 μs. After the electroporating pulse the membrane conductance begins to increase after a variable time delay (latency time) and shows two distinct phases, a first phase in which conductance increases very rapidly followed by a second slow phase. The inter-relationships between breakdown voltage, latency time and the membrane conductance were investigated in 25 different bilayers

Keywords

lipid bilayers; 10 mus; 300 to 600 mV; 500 mus; artificial lipid membranes electroporation; breakdown voltage; latency time; membrane conductance; negative slope ramp; rectangular voltage clamp pulse; variable time delay; Biomedical engineering; Biomembranes; Capacitance; Clamps; Current measurement; Delay; Lipidomics; Pulse measurements; USA Councils; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE

Conference_Location

Baltimore, MD

Print_ISBN

0-7803-2050-6

Type

conf

DOI

10.1109/IEMBS.1994.415380

Filename

415380