Immunomodulatory activity of herb-partition moxibustion on Stat3 and Stat4 in the mucosa of ulcerative colitis rats

Author

Zhou, En-Hua ; Liu, Hui-Rong ; Wang, Xiao-Mei ; Tan, Lin-Ying ; Wu, Huan-gan ; Shi, Yin ; Xu, Shi-fen ; Qin, Xiu-Di ; Huang, Wen-Yan

Author_Institution

Fudan Univ., Shanghai, China

fYear

2010

fDate

18-18 Dec. 2010

Firstpage

660

Lastpage

665

Abstract

A rat model of ulcerative colitis (UC) was established using an immunological method involving local stimulation. After the UC rat model was established, HPM was administered to the UC rats (n = 8) for seven days. UC rats without HPM (n = 8) and normal rats (n = 8) were used as the controls. Hematoxylin and eosin staining was used to visualize the pathological changes in the rats´ colonic mucosa. The expression of Stat3 and Stat4 mRNAs in the colonic mucosa was detected with real-time fluorescent quantitative polymerase chain reaction. HPM ameliorated colonic mucosal damage in the UC model rats. HPM downregulated the expression of Stat4 mRNA in the colons of UC rats. HPM had no effect on the expression of Stat3 mRNA. HPM ameliorates the colonic mucosal damage in the UC rat model. Stat4 signaling is involved in the mechanism of HPM action.

Keywords

biomedical engineering; diseases; macromolecules; organic compounds; physiological models; Stat3; Stat4; ameliorated colonic mucosal damage; colonic mucosa; eosin; fluorescent quantitative polymerase chain reaction; hematoxylin; herb-partition moxibustion; immunomodulatory activity; mRNA; rat model; ulcerative colitis rats; immunomodulatory activity; moxibustion; mucosa;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedicine Workshops (BIBMW), 2010 IEEE International Conference on

Conference_Location

Hong, Kong

Print_ISBN

978-1-4244-8303-7

Electronic_ISBN

978-1-4244-8304-4

Type

conf

DOI

10.1109/BIBMW.2010.5703883

Filename

5703883