DocumentCode :
2387188
Title :
Dielectric properties of proteins encapsulated in silica gel
Author :
Bridelli, M.G. ; Capelletti, R. ; Mora, C. ; Pirola, L. ; Ronda, L.
Author_Institution :
Dipt. di Fisica, Parma Univ., Italy
fYear :
2002
fDate :
2002
Firstpage :
267
Lastpage :
270
Abstract :
The study of the interactions between a silicate gel matrix and proteins encapsulated in such porous materials may help to get further insight on the distribution of water molecules around biological macromolecules experiencing a quasi-physiological environments. In the present paper the Thermally Stimulated Depolarization Currents (TSDC) spectra of tetramethoxysilane based silica gel at different hydration degrees (h=25 to 0. 15 gwater/gdry material) have been monitored in the range 100-300 K, then analyzed and compared with those recorded for myoglobin, lysozyme and lipase embedded in the same matrix. The results obtained allow to identify up to three energetically distinct families of water molecules trapped in the gel network and, in the spectra of encapsulated proteins, to distinguish the contributions coming from the protein aqueous microenvironment and from the water dipoles directly related to the included protein structure.
Keywords :
gels; porous materials; proteins; thermally stimulated currents; 100 to 300 K; biological macromolecule; dielectric properties; encapsulated protein; hydration degree; lipase; lysozyme; myoglobin; porous material; quasi-physiological environment; tetramethoxysilane silica gel; thermally stimulated depolarization current spectra; water molecule distribution; Biochemistry; Biological materials; Biomedical monitoring; Biomedical optical imaging; Dielectrics; Molecular biophysics; Physics; Proteins; Silicon compounds; Temperature;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Electrets, 2002. ISE 11. Proceedings. 11th International Symposium on
Print_ISBN :
0-7803-7560-2
Type :
conf
DOI :
10.1109/ISE.2002.1042994
Filename :
1042994
Link To Document :
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