QD barcodes for biosensing and detection

Multiplexed nanobarcodes have been prepared with quantum dots (QDs) and amphiphilic copolymers consisted of hydrocarbons and maleic anhydride groups. In homogenous solution, the QD-polymer complexes self-assemble into nanobeads with narrow size dispersity, which has been previously achieved only for micrometer-sized beads in the presence of solid supports. More than 250 QDs can be loaded into a nanobead of 100 nm in diameter. Using this new generation of nanoprobe, sensitive detection of human prostate specific antigen (PSA) has also been demonstrated. This new technology is expected to open new opportunities in nanoparticle-based ultrasensitive and multiplexed detection and sensing.