Ebp1 expression is induced by influenza virus infection

Author

Ejima, Miho ; Ueda, Ryuta ; Kume, Shinichiro ; Okazaki, Daisuke ; Yamakawa, Takefumi ; Shiku, Hitoshi ; Honda, Ayae

Author_Institution

Dept. Frontier Biosci., Hosei Univ., Tokyo

fYear

2008

fDate

6-9 Nov. 2008

Firstpage

214

Lastpage

218

Abstract

Influenza virus PB1 protein plays the key role for the assembly and catalytic function of viral RNA polymerase. We showed that Ebp1 interacted with PB1 and interfered both the viral RNA polymerase function in vitro and the virus growth. The Ebp1 expression was induced by influenza virus infection using western blotting and quantitative real time PCR assay. However, the quantitative determination has been requested of the relationship between influenza virus growth and Ebp1 level. To analyze the relationship between virus growth and Ebp1 level, we developed the assay system of single cell. Using this system, we confirmed the tight correlation between the Ebp1 level and the influenza virus growth.

Keywords

biochemistry; catalysis; cellular biophysics; enzymes; microorganisms; molecular biophysics; Ebp1 expression; PB1 protein; assembly function; catalytic function; cellular biophysics; influenza virus infection; quantitative real time PCR assay; viral RNA polymerase function; virus growth; vitro growth; western blotting; Assembly; Biochemistry; Bioinformatics; Genomics; In vitro; Influenza; Insects; Polymers; Proteins; RNA;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4244-2918-9

Electronic_ISBN

978-1-4244-2919-6

Type

conf

DOI

10.1109/MHS.2008.4752452

Filename

4752452