Title :
Single Live-Bacterial Cell Assay of Promoter Activity and Regulation
Author :
Teramoto, Jun ; Hasegawa, Akiko ; Shohata, Eri ; Kori, Ayako ; Magdy, El-Shimy H. ; Nakajima, Masahiro ; Lin, Yu-Ching ; Yamanishi, Yoko ; Magariyama, Yukio ; Arai, Fumihito ; Fukuda, Toshlo ; Ishihama, Akira
Author_Institution :
Dept. of Frontier Biosci., Hosei Univ., Koganei
Abstract :
For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture using the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of E. coli gcl promoter strength.
Keywords :
microorganisms; molecular biophysics; E coli gcl promoter strength; promoter activity; promoter regulation; single live-bacterial cell assay; Bioinformatics; Biomedical engineering; Cells (biology); Genomics; Indium tin oxide; Microorganisms; Semiconductor device measurement; Size control; Temperature measurement; Temperature sensors;
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-2918-9
Electronic_ISBN :
978-1-4244-2919-6
DOI :
10.1109/MHS.2008.4752467
